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Second Thermal and Fluids Engineering  Conference

ISSN: 2379-1748


Kewei Shi
Memorial University of Newfoundland

Fengmin Su
Marine Engineering College, Dalian Maritime University, Dalian, Liaoning 116026 China

Hongbin Ma
Marine Engineering College, Dalian Maritime University, Dalian, Liaoning 116026 China; Department of Mechanical and Aerospace Engineering, University of Missouri, Columbia, Missouri, 65211, USA

Xili Duan
Faculty of Engineering and Applied Science, Memorial University of Newfoundland, 230 Elizabeth Ave, St. John's, NL A1B 3X5, Canada

DOI: 10.1615/TFEC2017.mst.017617
pages 2807-2813


Vitrification is one of the most effective methods for cell cryopreservation. An ultra-fast cooling rate is beneficial to improve the vitrification level of the extra- and intra-cellular solution and decrease the concentration of cryoprotectant. This article focuses on the evaporation characteristics of liquid nitrogen on a micro structured surface (MSS) etched into the frozen carrier surface. Three main parameters, the heat flux, the total heat resistance and the evaporation heat transfer coefficient of the liquid nitrogen, were investigated in the dangerous temperature zone (0°C ~ - 60°C) using a lumped capacitance method. The experimental work verified the feasibility of fast freezing based on liquid nitrogen thin film evaporation. The heat transfer characteristics of the frozen carrier were experimentally measured and analyzed. It was found that the frozen carrier and the fast cooling technology designed in this study can acquire a high average cooling rate of 9.04 × 104 °C/min in the 0°C ~ - 60°C temperature zone. Two types of frozen carriers, an etched micro structure on the surface (MSS) and a plain surface, were compared: both carriers showed optimal heat transfer performance at a pressure of 10.3kPa, but the carrier with etched MSS achieved 44% higher average cooling rate than the one with plain surface.

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