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Second Thermal and Fluids Engineering  Conference

ISSN: 2379-1748
ISBN: 978-1-56700-430-4

Numerical and Experimental Study on the Detection Limit and Detection Range on the Performance of Lateral Flow Assays

Zhi Liu
Key Laboratory of Thermo-Fluid Science and Engineering of Ministry of Education, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, P.R. China

Zhiguo Qu
MOE Key Laboratory of Thermal-Fluid Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, China

Abstract

Lateral flow assays (LFAs) have drawn considerable research interest in point-of-care testing because of their rapidity, convenience and cost-effectiveness. However, they have been confined by the poor sensitivity and the difficulty to achieve quantitative or semi-quantitative detection. To address this, we use Hook effect tendency to give a synthetic evaluation on the effects of influence factors (e.g., capture probe concentration and report particle concentration) on the detection limit and detection range of LFAs by numerical and experimental methods. We observe that both higher capture probe concentration and report particle concentration will produce lower detection limit of LFAs and wider linear detection range as shown in both experimental data and simulation result. The current investigation provides a physically and synthetically intuitive illustration of fundamental of LFAs and could help to optimize the design of more sensitive LFAs and realize semi-quantitative detection.

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