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ISSN Online: 2379-1748

ISBN Flash Drive: 978-1-56700-483-0

ISBN Online: 978-1-56700-482-3

4th Thermal and Fluids Engineering Conference
April, 14–17, 2019 , Las Vegas, NV, USA

NUMERIC STUDY OF FREEZING TO SUPPORT DEVELOPMENT OF INDIVIDUAL CELL-SCALE THERMAL ANALYZER

Get access (open in a dialog) pages 803-816
DOI: 10.1615/TFEC2019.bio.028075

要約

It is well known that ice crystal formation within biological tissues and cells limits the survival potential for cryopreservation. By managing the formation of ice crystals, successful cryopreservation protocols have been developed for limited biological structures. It is believed that a better understanding of the ice formation process will lead to a broader application of cryopreservation techniques.
A latent heat measurement tool has been under development for individual cells to analyze the phase change for a range of potential cryoprotectants. The intent is also for the tool to be able to determine the amount of ice that forms during a freezing process. In order to better develop that tool, a series of numeric simulations have been carried out to determine the available signal expected for a freezing cell.
Transient numeric simulations were carried out using the commercial code Star-CCM+ on representatively-sized water drops. The numeric model was calibrated using measurements from a prototypical experiment using water droplets as a biological cell surrogate. The validated model was used to conduct a parametric study on cell-sized water drops to determine the expected spatial temperature response. The parametric study included phase change associated with the freezing of the entire water mass, as well as fractions of the mass. The purpose for studying fractional freezing is to determine the extent to which the temperature response at the location of a temperature is sensitive to where the freezing portion location. The results suggest that the freezing location does indeed influence that response.