ISSN Online: 2379-1748
ISBN Flash Drive: 978-1-56700-469-4
ISBN Online: 978-1-56700-470-0
Second Thermal and Fluids Engineering Conference
KRIOBLAST™: A PROMISING CRYOGENIC PLATFORM FOR KINETIC VITRIFICATION BY HYPERFATST COOLING
Аннотация
Cryopreservation and cryobanking are insurmountable parts of many sciences and related industries and technologies such as reproductive biology and animal husbandry, husbandry stem cell research and regenerative medicine and, cancer research and precision medicine. Kinetic (very rapid) vitrification (K-VF) is a promising approach for cryopreservation (CP) of biological materials as it is simple, robust, and can
achieve VF for practically any type of cells. Several methods of superfast K-VF, particularly for CP of
oocytes, embryos, sperm and human embryonic stem cells have been proposed but practically all of them
either require very small (in range 0.5-10 μL) size of the sample or/and cannot avoid the Leidenfrost effect
(LFE), which substantially impedes the rate of cooling. Here, we are reporting an entirely novel system is
based on hyperfast spray cooling of a hermetic cryocontainer for K-VF of one-two order of magnitude larger
(thousands of microliters) samples, which we called KrioBlastTM. The system completely eliminates LFE and a need of potentially toxic and osmotically damaging vitrificants such as dimethyl sulfoxide or ethylene
glycol used in the current methods of VF. We have been able to stably (repeatedly) vitrify up to 4,000 μL of
15% glycerol solution (used as a cooling rate marker), which theoretically corresponds to the critical cooling
rate up to 10,000 °K/s. This platform can be considered as a step toward the "Unified Cryopreservation
Protocol".